{
  "schemaVersion": 1,
  "capturedAt": "2026-10-29T10:31:13.717Z",
  "sha256": "c6828cd3d68c37c0fd7e7e03f36108eea17da16cfce36c11da8e555196f8d4ab",
  "work": {
    "type": "RDN",
    "kind": "note",
    "key": "miao-2024",
    "id": "TAP-RDN-0063",
    "locale": "zh",
    "path": "/zh/research/miao-2024/",
    "title": "TAP 导读: Brain clearance is reduced during sleep and anesthesia",
    "description": "该小鼠研究在所测状态下观察到相近的扩散系数，却在恢复睡眠及所用麻醉条件下估计到较低的示踪剂清除。结果提示需要把分子扩散、脑内分布和清除区分开，并对照具体实验方法理解睡眠研究。",
    "rights": "© The Allostasis Project。保留所有权利。除法律允许的例外外，转载或改编需另行授权。",
    "version": "1.0",
    "updatedAt": "2026-10-29",
    "editorial": {
      "contentType": "evidence-review",
      "reviewState": "draft",
      "reviewedAt": null,
      "reviewedVersion": null,
      "updatedAt": "2026-10-29",
      "version": "1.0",
      "byline": "team",
      "author": null,
      "reviewer": null,
      "workflow": {
        "ai": {
          "usage": "used",
          "system": "OpenAI Codex",
          "scope": "Assisted source comparison, evidence extraction and bilingual research-note preparation. The materials consulted and unresolved scientific issues are specified in each note.",
          "version": "1.0",
          "tasks": [
            "Source comparison and evidence extraction",
            "Bilingual research-note drafting and editorial comparison"
          ]
        }
      },
      "revisions": [
        {
          "date": "2026-10-29",
          "version": "1.0",
          "description": "新增2024年小鼠研究并配对图3b源数据更正，保留方法差异与复算范围。"
        }
      ]
    },
    "original": {
      "id": "miao-2024",
      "title": "Brain clearance is reduced during sleep and anesthesia",
      "authors": "Miao A · Luo T · Hsieh B · Edge CJ · Gridley M · Wong RTC · Constandinou TG · Wisden W · Franks NP",
      "journal": "Nature Neuroscience",
      "year": "2024",
      "url": "https://doi.org/10.1038/s41593-024-01638-y",
      "doi": "10.1038/s41593-024-01638-y",
      "pmid": "38741022"
    },
    "references": [
      {
        "id": "miao-2024",
        "title": "Brain clearance is reduced during sleep and anesthesia",
        "authors": "Miao A · Luo T · Hsieh B · Edge CJ · Gridley M · Wong RTC · Constandinou TG · Wisden W · Franks NP",
        "journal": "Nature Neuroscience",
        "year": "2024",
        "url": "https://doi.org/10.1038/s41593-024-01638-y",
        "doi": "10.1038/s41593-024-01638-y",
        "pmid": "38741022"
      }
    ],
    "sections": [
      {
        "id": "finding",
        "title": "研究发现",
        "paragraphs": [
          "该小鼠研究在所测状态下观察到相近的扩散系数，却在恢复睡眠及所用麻醉条件下估计到较低的示踪剂清除。结果提示需要把分子扩散、脑内分布和清除区分开，并对照具体实验方法理解睡眠研究。"
        ]
      },
      {
        "id": "limits",
        "title": "范围与局限",
        "paragraphs": [
          "3—7月龄雄性C57/BL6小鼠，不同扩散与清除测定使用不同子样本。",
          "睡眠是在5小时剥夺后的恢复睡眠；多数操作未盲，没有事前样本量计算，且存在拟合失败等排除。方法与2013年Xie研究不同。2024年正式更正修复图3b源数据Mean Wake 3h列；本导读未独立复算图3b，引用数据须使用出版社当前修正版。"
        ]
      },
      {
        "id": "sample",
        "title": "研究人群与样本",
        "paragraphs": [
          "3—7月龄雄性C57/BL6小鼠，不同扩散与清除测定使用不同子样本。"
        ]
      },
      {
        "id": "exposure",
        "title": "暴露或干预",
        "paragraphs": [
          "FITC-dextran光漂白测扩散，脑实质注射AF488后用荧光与模型估计清除；同鼠清醒/睡眠顺序随机。"
        ]
      },
      {
        "id": "outcomes",
        "title": "观察结局",
        "paragraphs": [
          "扩散系数与模型估计的示踪剂清除，依赖分布和距离假设。"
        ]
      },
      {
        "id": "duration",
        "title": "时间范围",
        "paragraphs": [
          "急性清醒、剥夺后恢复睡眠及麻醉观察。"
        ]
      },
      {
        "id": "funding",
        "title": "研究资金来源",
        "paragraphs": [
          "Wellcome 220759/Z/20/Z；UK Dementia Research Institute UK DRI-5004；EPSRC与MRC学生资助；成像设施获Wellcome 104931/Z/14/Z及BBSRC BB/L105129/1支持。"
        ]
      },
      {
        "id": "conflicts",
        "title": "研究利益关系",
        "paragraphs": [
          "作者声明无竞争性利益。"
        ]
      },
      {
        "id": "basis",
        "title": "导读范围",
        "paragraphs": [
          "全文主结果、动物/清除/统计/排除方法、图注及正式更正；本地源数据与出版社当前修正版的逐项比对仍未完成。"
        ],
        "links": [
          {
            "label": "PubMed Central全文",
            "url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC11156584/"
          },
          {
            "label": "正式更正：图3b源数据",
            "url": "https://www.nature.com/articles/s41593-024-01698-0"
          },
          {
            "label": "对照阅读相关原始研究及其方法",
            "url": "https://doi.org/10.1126/science.1241224"
          }
        ]
      }
    ]
  }
}